HIV (group M, subtype B, isolate HXB2) Gag-Pol polyProtein, His Tag_天津卡梅德科技发展有限公司

>>

>>

>>

>

HIV (group M, subtype B, isolate HXB2) Gag-Pol polyProtein, His Tag

HIV (group M, subtype B, isolate HXB2) Gag-Pol polyProtein, His Tag

产品编号

KMP4803

别名

人类免疫缺陷病毒Gag-Pol多聚蛋白, HIV Gag-Pol polyProtein, Gag-Pol poly, HIV Gag-Pol polyProtein Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Alias	人类免疫缺陷病毒Gag-Pol多聚蛋白, HIV Gag-Pol polyProtein, Gag-Pol poly, HIV Gag-Pol polyProtein Protein
Catalog Number	KMP4803
Product Description	The HIV (group M, subtype B (isolate HXB2) Gag-Pol polyProtein(KMP4803) is produced in E. coli. A DNA sequence encoding the p51 (Reverse Transcriptase)of human immunodeficiency virus 1 gag-pol (NP_057849.4) (Pro588-Phe1027) was expressed with a polyhistidine tag at the N-terminus.
Molecular Name	HIV Gag-Pol polyProtein
Species	HIV
Host	E. coli
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-Gag-Pol多聚蛋白的功能是什么？

包含结构蛋白（Gag）和酶类（Pol，如逆转录酶、整合酶），是病毒组装和复制的核心多蛋白前体。
Q2-该蛋白是否具有自剪切活性？

需要病毒蛋白酶（如HIV-1 Protease）切割才能释放功能性亚基，单独表达时以未切割形式存在。
Q3-能否用于体外病毒组装研究？

是的，与基因组RNA及脂质膜共孵育可模拟病毒颗粒组装过程。
Q4-His标签的定位是否影响功能？

标签位于N或C末端（需确认设计），经验证不影响蛋白酶切割位点及蛋白功能。
Q5-如何检测Gag-Pol的表达完整性？

通过Western Blot使用抗Gag或抗Pol抗体验证各结构域的存在。

推荐产品

400-621-6806
614353794
周一至周五 9:00-18:00
0