HIV (group M, subtype A, strain 92UG037.1) gp140 Protein, Fc Tag_天津卡梅德科技发展有限公司

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HIV (group M, subtype A, strain 92UG037.1) gp140 Protein, Fc Tag

HIV (group M, subtype A, strain 92UG037.1) gp140 Protein, Fc Tag

产品编号

KMP4801

别名

人类免疫缺陷病毒包膜蛋白gp140, HIV gp140, Envelope Protein gp140, HIV gp140 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	人类免疫缺陷病毒包膜蛋白gp140, HIV gp140, Envelope Protein gp140, HIV gp140 Protein
Catalog Number	KMP4801
Product Description	The HIV (group M, subtype A, strain 92UG037.1) gp140 Protein(KMP4801) is produced in HEK293 Cells. A DNA sequence encoding the HIV-1 envelope glycoprotein gp160 (AAC97548.1) extracellular domain (Leu33-Lys659), termed as gp140, was expressed with the Fc region of human IgG1 at the C-terminus.
Molecular Name	HIV gp140
Species	HIV
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-gp140与gp120/gp41有何区别？

gp140是gp120和gp41的截短融合形式，保留天然三聚体构象，更稳定且免疫原性更强，常用于疫苗开发。
Q2-Fc标签的作用是什么？

Fc标签可延长蛋白半衰期（通过结合新生儿Fc受体），并简化Protein A/G纯化流程。
Q3-该毒株的亚型A有何特点？

亚型A在东非和东欧广泛流行，研究其gp140可为区域性疫苗设计提供抗原模板。
Q4-如何验证三聚体结构？

通过凝胶过滤层析（SEC-HPLC）和负染电镜确认其均一的三聚体形态。
Q5-是否适用于黏膜免疫研究？

是的，结合黏膜佐剂（如霍乱毒素B亚基），可通过鼻内或口服途径评估黏膜IgA应答。

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