HCoV-HKU1 (isolate N1) Spike S2 Protein, His Tag_天津卡梅德科技发展有限公司

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HCoV-HKU1 (isolate N1) Spike S2 Protein, His Tag

HCoV-HKU1 (isolate N1) Spike S2 Protein, His Tag

产品编号

KMP4800

别名

人类冠状病毒刺突蛋白S2, HCoV-HKU1 Spike S2, S2 Protein, HCoV-HKU1 Spike S2 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	人类冠状病毒刺突蛋白S2, HCoV-HKU1 Spike S2, S2 Protein, HCoV-HKU1 Spike S2 Protein
Catalog Number	KMP4800
Product Description	The HCoV-HKU1 (isolate N1) Spike S2 Protein(KMP4800) is produced in Insect Cells. A DNA sequence encoding the Human coronavirus HKU1 (isolate N1) (HCoV-HKU1) Spike S2 (YP_173238.1)(Ser761-Pro1300) was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	HCoV-HKU1 Spike S2
Species	HCoV-HKU1
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 300mM NaCl, 10% Glycerol, pH8.0
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-Spike S2蛋白的功能是什么？

S2亚基介导病毒与宿主细胞膜融合，是冠状病毒进入宿主的重要步骤，也是广谱抗病毒药物的潜在靶点。
Q2-HKU1 N1分离株的研究意义是什么？

HCoV-HKU1可导致人类呼吸道感染，研究S2蛋白有助于理解其传播机制及开发交叉保护性疫苗。
Q3-该蛋白是否保留膜融合活性？

是的，通过脂质体融合实验验证其可诱导膜融合，活性依赖pH值变化（需酸性环境激活）。
Q4-His标签的纯化效率如何？

经镍柱亲和层析纯化后，纯度≥90%（SDS-PAGE验证），适用于结构生物学研究。
Q5-能否用于抗体交叉反应研究？

是的，可测试针对其他冠状病毒（如SARS-CoV-2）S2抗体的交叉中和能力。

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