HIV (group M, subtype CRF07_BC) gp120 Protein, His Tag_天津卡梅德科技发展有限公司

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HIV (group M, subtype CRF07_BC) gp120 Protein, His Tag

HIV (group M, subtype CRF07_BC) gp120 Protein, His Tag

产品编号

KMP4799

别名

人类免疫缺陷病毒包膜蛋白gp120, HIV gp120, Envelope Protein gp120, HIV gp120 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	人类免疫缺陷病毒包膜蛋白gp120, HIV gp120, Envelope Protein gp120, HIV gp120 Protein
Catalog Number	KMP4799
Product Description	The HIV (group M, subtype CRF07_BC) gp120 Protein(KMP4799) is produced in HEK293 Cells. A DNA sequence encoding the HIV-1 envelope glycoprotein gp120 (AFM77980.1) (Ala29-Glu502) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.
Molecular Name	HIV gp120
Species	HIV
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 150mM NaCl, 0.2M Arg, 10% Glycerol, 0.01% Tween20, pH8.5
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-gp120蛋白在HIV感染中的作用是什么？

gp120是HIV包膜糖蛋白，负责结合宿主CD4受体和趋化因子共受体（如CCR5/CXCR4），是病毒入侵宿主细胞的关键分子。
Q2-CRF07_BC亚型有何流行病学特征？

CRF07_BC是中国及东南亚地区主要流行的HIV-1重组型，研究其gp120有助于开发区域性疫苗和诊断工具。
Q3-His标签如何影响gp120的功能？

His标签位于C末端，不影响gp120的受体结合活性和构象，可通过镍柱纯化并通过抗His抗体检测。
Q4-该蛋白适用于哪些研究？

用于中和抗体筛选、受体结合机制分析、疫苗抗原设计及HIV血清学检测。
Q5-如何验证gp120的糖基化修饰？

通过质谱分析确认N-糖基化位点（如Asn-X-Ser/Thr），并与天然病毒颗粒的糖基化模式对比。

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