H5N1 (A/Cambodia/NPH230032/2023) NP Protein, His Tag_天津卡梅德科技发展有限公司

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H5N1 (A/Cambodia/NPH230032/2023) NP Protein, His Tag

H5N1 (A/Cambodia/NPH230032/2023) NP Protein, His Tag

产品编号

KMP4792

别名

甲型流感病毒H5N1核蛋白, H5N1 NP, Nucleoprotein, H5N1 NP Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H5N1核蛋白, H5N1 NP, Nucleoprotein, H5N1 NP Protein
Catalog Number	KMP4792
Product Description	The H5N1 (A/Cambodia/NPH230032/2023) NP Protein(KMP4792) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus ((A/Cambodia/NPH230032/2023) (H5N1)) nucleoprotein (EPI2419703) (Met1-Asn498) was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H5N1 NP
Species	H5N1
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-NP蛋白在病毒复制中的作用是什么？

NP（核蛋白）包裹病毒RNA形成核糖核蛋白复合体（RNP），是病毒复制和转录的核心元件。
Q2-柬埔寨2023株H5N1的威胁性如何？

该株属于高致病性禽流感病毒（HPAI），具有跨物种感染人类的风险，NP蛋白研究有助于追踪其进化路径。
Q3-该蛋白是否适用于T细胞免疫研究？

是的，NP是细胞毒性T淋巴细胞（CTL）的主要靶抗原，可用于评估交叉免疫应答。
Q4-如何检测蛋白纯度及宿主残留？

SDS-PAGE验证纯度≥90%，ELISA检测宿主细胞蛋白残留<0.05%。
Q5-是否支持病毒RNP重建实验？

需与PB1、PB2、PA及病毒RNA共孵育，NP是体外组装功能性RNP复合体的必需组分。

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