| Alias | 甲型流感病毒H1N1血凝素蛋白, H1N1 HA, Hemagglutinin Protein, H1N1 HA Protein |
| Catalog Number | KMP4786 |
| Product Description | The H1N1 (A/Wisconsin/67/2022) HA Protein(KMP4786) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus ((A/Wisconsin/67/2022) (H1N1)) hemagglutinin (translated amino acids of EPI2224788) (Met1-Ile530), termed as HA, was expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus. |
| Molecular Name | H1N1 HA |
| Species | H1N1 |
| Host | Insect Cells |
| Size | 50ug, 100ug, 200ug |
| Purity | >85% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | 20mM Tris, 150mM NaCl, 0.2M Arg, 10% Glycerol, 0.01% Tween20, pH8.5 |
| Background | The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity. |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
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