H6N1 (A/quail/Hong Kong/1721-30/99) HA Protein, His Tag_天津卡梅德科技发展有限公司

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H6N1 (A/quail/Hong Kong/1721-30/99) HA Protein, His Tag

H6N1 (A/quail/Hong Kong/1721-30/99) HA Protein, His Tag

产品编号

KMP4613

别名

甲型流感病毒H6N1血凝素蛋白, H6N1 HA, Hemagglutinin Protein, H6N1 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H6N1血凝素蛋白, H6N1 HA, Hemagglutinin Protein, H6N1 HA Protein
Catalog Number	KMP4613
Product Description	The H6N1 (A/quail/Hong Kong/1721-30/99) HA Protein(KMP4613) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/quail/Hong Kong/1721-30/99 (H6N1)) hemagglutinin (CAC84981.1) (Met1-Arg345), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H6N1 HA
Species	H6N1
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>85% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 500mM NaCl, 10% Glycerol, pH8.0
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-鹌鹑来源的H6N1病毒有何特殊性？

鹌鹑是禽流感病毒从野鸟向家禽传播的“桥梁宿主”，其HA蛋白研究有助于解析跨物种适应机制。
Q2-该HA蛋白是否具有跨膜结构域？

本产品为可溶性截短体，去除跨膜区（保留HA1+HA2胞外域），增强溶解性。
Q3-能否用于假病毒包装？

是的，与慢病毒载体共转染可生成假病毒，用于中和抗体检测或宿主嗜性研究。
Q4-是否验证过与哺乳动物细胞的结合能力？

通过流式细胞术验证其可结合表达α-2,3/α-2,6唾液酸受体的MDCK细胞系。
Q5-运输过程中如何保持活性？

干冰运输，到货后立即-80℃保存，避免反复冻融（建议分装使用）。

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