H4N2 (A/duck/Hunan/8-19/2009) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H4N2 (A/duck/Hunan/8-19/2009) HA1 Protein, His Tag

H4N2 (A/duck/Hunan/8-19/2009) HA1 Protein, His Tag

产品编号

KMP4608

别名

甲型流感病毒H4N2血凝素蛋白1, H4N2 HA1, Hemagglutinin Protein, H4N2 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H4N2血凝素蛋白1, H4N2 HA1, Hemagglutinin Protein, H4N2 HA1 Protein
Catalog Number	KMP4608
Product Description	The H4N2 (A/duck/Hunan/8-19/2009) HA1 Protein(KMP4608) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/duck/Hunan/8-19/2009(H4N2)) hemagglutinin (ADN78261.1) (Met1-Arg343), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H4N2 HA1
Species	H4N2
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-HA1与全长HA的应用场景有何不同？

HA1聚焦于受体结合域研究，适用于表位作图、抗体中和机制分析，而全长HA更适用于膜融合或疫苗研究。
Q2-该蛋白是否保留天然糖基化修饰？

在HEK293细胞中表达，保留与天然病毒一致的糖基化修饰，经PNGase F酶切验证。
Q3-能否用于单克隆抗体的高通量筛选？

是的，可固相化于芯片或磁珠，结合噬菌体展示文库筛选高亲和力抗体。
Q4-溶解后蛋白浓度如何测定？

推荐BCA法或紫外分光光度法（A280，消光系数1.1 L/(g·cm)），避免SDS干扰。
Q5-是否提供冻干保护剂配方？

冻干粉含海藻糖和甘露醇（质量比1:1），复溶后稳定性高。

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