H10N8 (A/duck/Guangdong/E1/2012) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H10N8 (A/duck/Guangdong/E1/2012) HA1 Protein, His Tag

H10N8 (A/duck/Guangdong/E1/2012) HA1 Protein, His Tag

产品编号

KMP4599

别名

甲型流感病毒H10N8血凝素蛋白1, H10N8 HA1, Hemagglutinin Protein, H10N8 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H10N8血凝素蛋白1, H10N8 HA1, Hemagglutinin Protein, H10N8 HA1 Protein
Catalog Number	KMP4599
Product Description	The H10N8 (A/duck/Guangdong/E1/2012) HA1 Protein(KMP4599) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/duck/Guangdong/E1/2012(H10N8)) hemagglutinin (Met1-Arg340), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H10N8 HA1
Species	H10N8
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 500mM NaCl, 10% Glycerol, pH8.0
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA1蛋白的流行病学意义是什么？

该毒株分离自中国广东的鸭群，H10N8亚型曾导致人类感染，研究其HA1可揭示跨物种传播的分子基础及潜在公共卫生风险。
Q2-HA1的受体结合特性是否偏好人类或禽类受体？

实验表明其优先结合禽源α-2,3唾液酸受体，但需通过糖芯片或SPR进一步验证对人源α-2,6受体的亲和力。
Q3-该蛋白能否用于H10N8特异性诊断试剂开发？

是的，可作为ELISA或免疫层析试剂的抗原，检测患者血清中的H10亚型抗体。
Q4-是否提供糖基化修饰分析？

可提供基于质谱的N-糖基化位点鉴定报告（如Asn-X-Ser/Thr），需额外订购。
Q5-如何避免蛋白聚集？

建议复溶时轻柔涡旋，避免剧烈震荡，溶液中含0.01% Tween-20以增强稳定性。

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