H3N2 (A/Aichi/2/1968) Matrix Protein, His Tag_天津卡梅德科技发展有限公司

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H3N2 (A/Aichi/2/1968) Matrix Protein, His Tag

H3N2 (A/Aichi/2/1968) Matrix Protein, His Tag

产品编号

KMP4591

别名

甲型流感病毒H3N2基质蛋白, H3N2 Matrix, VP40, H3N2 Matrix Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H3N2基质蛋白, H3N2 Matrix, VP40, H3N2 Matrix Protein
Catalog Number	KMP4591
Product Description	The H3N2 (A/Aichi/2/1968) Matrix Protein(KMP4591) is produced in E. coli. A DNA sequence encoding the influenza A virus (A/Aichi/2/1968(H3N2)) matrix protein 1 (AFM71858.1) (Met1-Lys252), termed as M1, was fused with a N-terminal polyhistidine tag.
Molecular Name	H3N2 Matrix
Species	H3N2
Host	E. coli
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该M1蛋白与1918株M1的差异是什么？

Aichi/1968是季节性H3N2的代表株，其M1氨基酸序列与1918株存在约20%差异，反映长期进化中的适应性突变。
Q2-能否用于抗病毒药物筛选？

是的，M1参与病毒组装，可作为靶点筛选抑制病毒出芽的小分子化合物。
Q3-蛋白的溶解性如何？

在pH 7.4缓冲液中溶解性良好（>1 mg/mL），避免反复冻融以防聚集。
Q4-是否支持单克隆抗体制备？

是的，已成功用于小鼠免疫实验，产生高滴度M1特异性抗体。
Q5-如何验证蛋白的寡聚状态？

通过动态光散射（DLS）和凝胶过滤层析确认其以单体/二聚体形式存在。

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