H3N2 (A/Aichi/2/1968) NP Protein, His Tag_天津卡梅德科技发展有限公司

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H3N2 (A/Aichi/2/1968) NP Protein, His Tag

H3N2 (A/Aichi/2/1968) NP Protein, His Tag

产品编号

KMP4588

别名

甲型流感病毒H3N2核蛋白, H3N2 NP, Nucleoprotein, H3N2 NP Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H3N2核蛋白, H3N2 NP, Nucleoprotein, H3N2 NP Protein
Catalog Number	KMP4588
Product Description	The H3N2 (A/Aichi/2/1968) NP Protein(KMP4588) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/Aichi/2/1968(H3N2)) nucleoprotein (AFM71861.1) (Met1-Asn498) was expressed with a C-terminal polyhistidine tag.
Molecular Name	H3N2 NP
Species	H3N2
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 150mM NaCl, 10% Glycerol, pH8.0
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-NP蛋白在病毒生命周期中的作用是什么？

NP（核蛋白）包裹病毒RNA形成核糖核蛋白复合体（RNP），是病毒复制和转录的核心组件。
Q2-该蛋白是否适合研究宿主免疫应答？

是的，NP是细胞毒性T淋巴细胞（CTL）的主要靶抗原，可用于评估交叉免疫保护。
Q3-能否用于病毒颗粒的体外组装实验？

需结合其他病毒蛋白（如M1、RNA），但NP是体外重建RNP复合体的必需组分。
Q4-蛋白的纯化策略是什么？

通过镍柱亲和层析纯化，并经离子交换层析去除核酸残留，纯度≥90%。
Q5-是否提供非变性条件下的储存形式？

蛋白溶于含10%甘油的PBS缓冲液（pH 7.4），-80℃保存可维持天然构象。

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