H7N7 (A/Netherlands/219/2003) NA Protein, His Tag_天津卡梅德科技发展有限公司

>>

>>

>>

>

H7N7 (A/Netherlands/219/2003) NA Protein, His Tag

H7N7 (A/Netherlands/219/2003) NA Protein, His Tag

产品编号

KMP4584

别名

甲型流感病毒H7N7神经氨酸酶, H7N7 NA, Neuraminidase Protein, H7N7 NA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Alias	甲型流感病毒H7N7神经氨酸酶, H7N7 NA, Neuraminidase Protein, H7N7 NA Protein
Catalog Number	KMP4584
Product Description	The H7N7 (A/Netherlands/219/2003) NA Protein(KMP4584) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/Netherlands/219/2003(H7N7)) neuraminidase (AAR11367.1) (His36-Ser471) was expressed with a polyhistidine tag at the N-terminus.
Molecular Name	H7N7 NA
Species	H7N7
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 500mM NaCl, 10% Glycerol, pH7.0
Background	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该NA蛋白的功能是什么？

NA（神经氨酸酶）是流感病毒表面蛋白，负责切割宿主细胞表面的唾液酸残基，促进病毒颗粒释放，是抗病毒药物（如奥司他韦）的主要靶点。
Q2-该蛋白是否具有酶活性？如何验证？

是的，可通过荧光底物（如MUNANA）检测其神经氨酸酶活性，活性单位（NAU）需参考标准曲线计算。
Q3-该毒株的流行病学意义是什么？

H7N7 (A/Netherlands/219/2003) 曾引起人类感染暴发，研究其NA蛋白有助于理解高致病性禽流感的传播机制。
Q4-His标签是否影响蛋白功能？

标签位于C末端，经活性验证表明不影响酶活性和抗原性。
Q5-该蛋白能否用于疫苗研发？

NA蛋白可作为疫苗候选成分，诱导免疫应答，但需与HA蛋白联合使用以提高保护效果。

推荐产品

400-621-6806
614353794
周一至周五 9:00-18:00
0