H15N2 (A/Australian shelduck/Western Australia/1756/1983) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H15N2 (A/Australian shelduck/Western Australia/1756/1983) HA1 Protein, His Tag

H15N2 (A/Australian shelduck/Western Australia/1756/1983) HA1 Protein, His Tag

产品编号

KMP4578

别名

甲型流感病毒H15N2血凝素蛋白1, H15N2 HA1, Hemagglutinin Protein, H15N2 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H15N2血凝素蛋白1, H15N2 HA1, Hemagglutinin Protein, H15N2 HA1 Protein
Catalog Number	KMP4578
Product Description	The H15N2 (A/Australian shelduck/Western Australia/1756/1983) HA1 Protein(KMP4578) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/Australian shelduck/ Western Australia/1756/1983(H15N2)) hemagglutinin (ABB90704.1) (ABB90704.1) (Met1-Arg349), termed as HA1, was expressed with a C-terminal polyhistidine tag.
Molecular Name	H15N2 HA1
Species	H15N2
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>85% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The HIV-1 gp120 envelope protein, a glycoprotein that is part of the outer layer of the virus, is an essential component in the multi-tiered viral entry process. It presents itself as viral membrane spikes consisting of 3 molecules of gp120 linked together and anchored to the membrane by gp41 protein. Gp120 is essential for viral infection as it facilitates HIV entry into the host cell and this is its best-known and most researched role in HIV infection. However, it is becoming increasingly evident that gp120 might also be facilitating viral persistence and continuing HIV infection by influencing the T cell immune response to the virus. The surface protein gp120 attaches the virus to the host lymphoid cell by binding to the primary receptor CD4. Gp120 binding to its receptor CD4 and co-receptor, CXCR4 or CCR5 is required for fusion of viral and cellular membranes. Several mechanisms might be involved in this process of which gp120 binding to the CD4 receptor of T cells is the best known and most important interaction as it facilitates viral entry into the CD4+ cells and their depletion, a hallmark of the HIV infection. Gp120 is shed from the viral membrane and accumulates in lymphoid tissues in significant amounts. Despite the overall genetic heterogeneity of the gp120 glycoprotein, the conserved CD4 binding site provides an attractive antiviral target. Interaction between gp120 and ITGA4/ITGB7 would allow the virus to enter GALT early in the infection, infecting and killing most of GALT's resting CD4+ T-cells. This T-cell depletion is believed to be the major insult to the host immune system leading to AIDS.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-HA1与全长HA蛋白的应用场景有何区别？

HA1适合表位精细定位和抗体表位作图，而全长HA更适用于膜融合或疫苗开发研究。
Q2-该蛋白是否含有宿主细胞蛋白残留？

经ELISA检测，宿主蛋白残留<0.1%，符合国际标准。
Q3-能否用于免疫印迹（Western Blot）？

是的，推荐使用抗His标签抗体（1:2000稀释）进行检测。
Q4-是否支持结构生物学合作研究？

可提供晶体筛选或冷冻电镜样品制备技术支持（需签订合作协议）。
Q5-复溶后蛋白浓度如何测定？

建议使用BCA法或紫外分光光度计（A280，消光系数为1.2 L/(g·cm)）定量。

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