H13N6 (A/black-headed gull/Sweden/1/1999) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

>>

>>

>>

>

H13N6 (A/black-headed gull/Sweden/1/1999) HA1 Protein, His Tag

H13N6 (A/black-headed gull/Sweden/1/1999) HA1 Protein, His Tag

产品编号

KMP4575

别名

甲型流感病毒H13N6血凝素蛋白1, H13N6 HA1, Hemagglutinin Protein, H13N6 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Alias	甲型流感病毒H13N6血凝素蛋白1, H13N6 HA1, Hemagglutinin Protein, H13N6 HA1 Protein
Catalog Number	KMP4575
Product Description	The H13N6 (A/black-headed gull/Sweden/1/1999) HA1 Protein(KMP4575) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/black-headed gull/Sweden/1/1999(H13N6)) hemagglutinin (AAV91213.1) (Met1-Arg342), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H13N6 HA1
Species	H13N6
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The HIV-1 gp120 envelope protein, a glycoprotein that is part of the outer layer of the virus, is an essential component in the multi-tiered viral entry process. It presents itself as viral membrane spikes consisting of 3 molecules of gp120 linked together and anchored to the membrane by gp41 protein. Gp120 is essential for viral infection as it facilitates HIV entry into the host cell and this is its best-known and most researched role in HIV infection. However, it is becoming increasingly evident that gp120 might also be facilitating viral persistence and continuing HIV infection by influencing the T cell immune response to the virus. The surface protein gp120 attaches the virus to the host lymphoid cell by binding to the primary receptor CD4. Gp120 binding to its receptor CD4 and co-receptor, CXCR4 or CCR5 is required for fusion of viral and cellular membranes. Several mechanisms might be involved in this process of which gp120 binding to the CD4 receptor of T cells is the best known and most important interaction as it facilitates viral entry into the CD4+ cells and their depletion, a hallmark of the HIV infection. Gp120 is shed from the viral membrane and accumulates in lymphoid tissues in significant amounts. Despite the overall genetic heterogeneity of the gp120 glycoprotein, the conserved CD4 binding site provides an attractive antiviral target. Interaction between gp120 and ITGA4/ITGB7 would allow the virus to enter GALT early in the infection, infecting and killing most of GALT's resting CD4+ T-cells. This T-cell depletion is believed to be the major insult to the host immune system leading to AIDS.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-黑头鸥来源的H13N6病毒有何生态学意义？

鸥类常与多种水禽互动，其HA1研究可揭示病毒在野生鸟类中的重组潜力。
Q2-该蛋白是否具有膜融合活性？

HA1仅含受体结合域，不包含HA2介导的膜融合功能，需全长HA蛋白进行融合研究。
Q3-是否适用于小鼠免疫实验？

是的，已通过动物实验验证免疫原性，佐剂推荐使用弗氏佐剂或铝佐剂。
Q4-生产批次间的一致性如何保证？

每批均进行SDS-PAGE、Western Blot和活性检测，确保批间差异<10%。
Q5-能否提供非还原电泳数据？

可应要求提供还原与非还原条件下的SDS-PAGE结果，确认二硫键正确形成。

推荐产品

400-621-6806
614353794
周一至周五 9:00-18:00
0