H12N3 (A/bar headed goose/Mongolia/143/2005) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H12N3 (A/bar headed goose/Mongolia/143/2005) HA1 Protein, His Tag

H12N3 (A/bar headed goose/Mongolia/143/2005) HA1 Protein, His Tag

产品编号

KMP4574

别名

甲型流感病毒H12N3血凝素蛋白1, H12N3 HA1, Hemagglutinin Protein, H12N3 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H12N3血凝素蛋白1, H12N3 HA1, Hemagglutinin Protein, H12N3 HA1 Protein
Catalog Number	KMP4574
Product Description	The H12N3 (A/bar headed goose/Mongolia/143/2005) HA1 Protein(KMP4574) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/bar headed goose/Mongolia/143/2005(H12N3)) hemagglutinin (ACV86810.1), (Met1-Arg342) termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H12N3 HA1
Species	H12N3
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The HIV-1 gp120 envelope protein, a glycoprotein that is part of the outer layer of the virus, is an essential component in the multi-tiered viral entry process. It presents itself as viral membrane spikes consisting of 3 molecules of gp120 linked together and anchored to the membrane by gp41 protein. Gp120 is essential for viral infection as it facilitates HIV entry into the host cell and this is its best-known and most researched role in HIV infection. However, it is becoming increasingly evident that gp120 might also be facilitating viral persistence and continuing HIV infection by influencing the T cell immune response to the virus. The surface protein gp120 attaches the virus to the host lymphoid cell by binding to the primary receptor CD4. Gp120 binding to its receptor CD4 and co-receptor, CXCR4 or CCR5 is required for fusion of viral and cellular membranes. Several mechanisms might be involved in this process of which gp120 binding to the CD4 receptor of T cells is the best known and most important interaction as it facilitates viral entry into the CD4+ cells and their depletion, a hallmark of the HIV infection. Gp120 is shed from the viral membrane and accumulates in lymphoid tissues in significant amounts. Despite the overall genetic heterogeneity of the gp120 glycoprotein, the conserved CD4 binding site provides an attractive antiviral target. Interaction between gp120 and ITGA4/ITGB7 would allow the virus to enter GALT early in the infection, infecting and killing most of GALT's resting CD4+ T-cells. This T-cell depletion is believed to be the major insult to the host immune system leading to AIDS.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-斑头雁作为候鸟对病毒传播的意义是什么？

斑头雁迁徙路线跨越欧亚大陆，可能促进H12N3的远距离传播，研究其HA1有助于监测病毒扩散。
Q2-该蛋白能否用于假病毒包装？

是的，HA1可与慢病毒载体结合，构建假病毒用于中和实验或药物筛选。
Q3-是否验证过与抗体的交叉反应性？

已验证与H12亚型多克隆抗体特异性结合，与其他亚型（如H7/H9）无交叉反应。
Q4-蛋白的缓冲液成分是什么？

溶于PBS（pH 7.4），含5%甘油和0.01% Tween-20，增强溶解稳定性。
Q5-能否用于免疫荧光实验？

是的，需先固定并透化细胞，推荐使用抗His标签一抗进行检测。

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