H11N2 (A/thick-billed murre/Newfoundland/031/2007) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H11N2 (A/thick-billed murre/Newfoundland/031/2007) HA1 Protein, His Tag

H11N2 (A/thick-billed murre/Newfoundland/031/2007) HA1 Protein, His Tag

产品编号

KMP4572

别名

甲型流感病毒H11N2血凝素蛋白1, H11N2 HA1, Hemagglutinin Protein, H11N2 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H11N2血凝素蛋白1, H11N2 HA1, Hemagglutinin Protein, H11N2 HA1 Protein
Catalog Number	KMP4572
Product Description	The H11N2 (A/thick-billed murre/Newfoundland/031/2007) HA1 Protein(KMP4572) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/thick-billed murre/Newfoundland/031/2007(H11N2)) (Met1-Arg342), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H11N2 HA1
Species	H11N2
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-厚嘴海鸦来源的HA1蛋白有何研究价值？

海鸦是海洋禽类，其携带的H11N2病毒反映了禽流感在海洋生态系统中的多样性，为跨宿主传播研究提供模型。
Q2-该蛋白是否可用于单克隆抗体制备？

是的，免疫动物后可筛选针对H11亚型HA1结构域的特异性单克隆抗体。
Q3-蛋白的纯化方法是什么？

通过镍亲和层析纯化，并经分子筛层析进一步去除聚集体，纯度≥95%。
Q4-是否支持定制化修饰（如荧光标记）？

可提供生物素、FITC或HRP标记服务（需额外协商）。
Q5-长期保存是否会降低活性？

-80℃保存条件下，活性可维持2年以上；避免反复冻融。

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