H9N5 (A/shorebird/DE/261/2003)HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H9N5 (A/shorebird/DE/261/2003)HA1 Protein, His Tag

H9N5 (A/shorebird/DE/261/2003)HA1 Protein, His Tag

产品编号

KMP4569

别名

甲型流感病毒H9N5血凝素蛋白1, H9N5 HA1, Hemagglutinin Protein, H9N5 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H9N5血凝素蛋白1, H9N5 HA1, Hemagglutinin Protein, H9N5 HA1 Protein
Catalog Number	KMP4569
Product Description	The H9N5 (A/shorebird/DE/261/2003)HA1 Protein(KMP4569) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/shorebird/DE/261/2003(H9N5)) hemagglutinin (ABB87950.1) (Met1-Arg338), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H9N5 HA1
Species	H9N5
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA1蛋白是否保留天然受体结合活性？

是的，HA1亚基在哺乳动物细胞中表达，保留天然糖基化修饰和受体结合能力，可通过红细胞凝集实验验证活性。
Q2-该蛋白适用于哪些免疫学研究？

可用于H9N5特异性抗体检测、中和抗体评价及疫苗抗原性研究。
Q3-与全长HA蛋白相比，HA1版本的优势是什么？

HA1更易纯化且稳定性高，适合高分辨率结构分析（如X射线晶体学或冷冻电镜）。
Q4-该蛋白是否含有内毒素？

经检测内毒素水平<1.0 EU/μg（LAL法），适用于细胞实验和体内研究。
Q5-如何确认蛋白的亚型特异性？

通过N末端测序和质谱分析验证，确保与H9N5毒株HA1序列一致。

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