H9N5 (A/shorebird/DE/261/2003) HA Protein, His Tag_天津卡梅德科技发展有限公司

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H9N5 (A/shorebird/DE/261/2003) HA Protein, His Tag

H9N5 (A/shorebird/DE/261/2003) HA Protein, His Tag

产品编号

KMP4568

别名

甲型流感病毒H9N5血凝素蛋白, H9N5 HA, Hemagglutinin Protein, H9N5 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H9N5血凝素蛋白, H9N5 HA, Hemagglutinin Protein, H9N5 HA Protein
Catalog Number	KMP4568
Product Description	The H9N5 (A/shorebird/DE/261/2003) HA Protein(KMP4568) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/shorebird/DE/261/2003(H9N5)) hemagglutinin (ABB87950.1) (Met1-Lys523), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H9N5 HA
Species	H9N5
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA蛋白的宿主（shorebird）有何特殊性？

源自岸禽类（如鸻鹬），此类鸟类是禽流感病毒的重要自然宿主，研究其HA有助于了解病毒多样性。
Q2-能否用于跨物种传播机制研究？

是的，H9亚型具有感染哺乳动物的潜力，其HA蛋白是研究宿主范围的关键靶点。
Q3-是否提供受体结合特异性数据？

可提供与α-2,3或α-2,6唾液酸受体的结合实验报告（需额外订购）。
Q4-该蛋白是否适合ELISA检测？

是的，已验证可用于禽流感抗体ELISA检测，包被浓度建议1-5μg/mL。
Q5-生产过程中是否添加内毒素？

内毒素水平<1.0 EU/μg（LAL法检测），符合体外研究标准。

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