H7N8 (A/mallard/Netherlands/33/2006) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H7N8 (A/mallard/Netherlands/33/2006) HA1 Protein, His Tag

H7N8 (A/mallard/Netherlands/33/2006) HA1 Protein, His Tag

产品编号

KMP4566

别名

甲型流感病毒H7N8血凝素蛋白1, H7N8 HA1, Hemagglutinin Protein, H7N8 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H7N8血凝素蛋白1, H7N8 HA1, Hemagglutinin Protein, H7N8 HA1 Protein
Catalog Number	KMP4566
Product Description	The H7N8 (A/mallard/Netherlands/33/2006) HA1 Protein(KMP4566) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/mallard/Netherlands/33/2006(H7N8)) hemagglutinin (translated amino acid of EPI182113) (Met1-Arg339), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H7N8 HA1
Species	H7N8
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 500mM NaCl, 10% Glycerol, pH8.0
Background	The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-HA1蛋白的功能特异性是什么？

HA1是HA蛋白的受体结合亚基，直接参与病毒与宿主细胞表面唾液酸受体的结合。
Q2-该蛋白是否适合中和抗体研究？

是的，HA1是中和抗体的主要靶点，可用于抗体效价评估及表位定位。
Q3-与全长HA相比，HA1的优势是什么？

HA1更易表达和纯化，且能避免HA2引起的蛋白聚集问题，适合结构生物学研究。
Q4-是否提供糖基化修饰？

在哺乳动物细胞中表达，保留天然糖基化修饰。
Q5-如何保存该蛋白？

建议-80℃长期保存，避免反复冻融，溶解后4℃可稳定1周。

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