H7N8 (A/mallard/Netherlands/33/2006) HA Protein, His Tag_天津卡梅德科技发展有限公司

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H7N8 (A/mallard/Netherlands/33/2006) HA Protein, His Tag

H7N8 (A/mallard/Netherlands/33/2006) HA Protein, His Tag

产品编号

KMP4565

别名

甲型流感病毒H7N8血凝素蛋白, H7N8 HA, Hemagglutinin Protein, H7N8 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H7N8血凝素蛋白, H7N8 HA, Hemagglutinin Protein, H7N8 HA Protein
Catalog Number	KMP4565
Product Description	The H7N8 (A/mallard/Netherlands/33/2006) HA Protein(KMP4565) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/mallard/Netherlands/33/2006(H7N8)) hemagglutinin (ACR59554.1) (Met1-Phe535) was expressed with a C-terminal polyhistidine tag.
Molecular Name	H7N8 HA
Species	H7N8
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA蛋白的宿主来源有何研究价值？

源自在荷兰野鸭中分离的H7N8病毒，有助于研究禽类流感病毒的生态分布及潜在人畜共患风险。
Q2-该产品与HA1亚基版本的区别是什么？

本产品为全长HA蛋白，包含HA1和HA2亚基，而HA1版本仅含受体结合域（HA1），两者适用于不同功能研究。
Q3-该蛋白能否用于疫苗研发？

是的，全长HA蛋白可模拟天然构象，是疫苗抗原设计的理想候选。
Q4-如何验证蛋白活性？

可通过红细胞凝集实验（HA Assay）验证其受体结合活性。
Q5-是否提供无菌过滤和冻干形式？

标准产品为冻干粉末，溶解后经0.22μm过滤，确保无菌。

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