H7N7 (A/equine/Kentucky/1a/1975) HA Protein, His Tag_天津卡梅德科技发展有限公司

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H7N7 (A/equine/Kentucky/1a/1975) HA Protein, His Tag

H7N7 (A/equine/Kentucky/1a/1975) HA Protein, His Tag

产品编号

KMP4564

别名

甲型流感病毒H7N7血凝素蛋白, H7N7 HA, Hemagglutinin Protein, H7N7 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H7N7血凝素蛋白, H7N7 HA, Hemagglutinin Protein, H7N7 HA Protein
Catalog Number	KMP4564
Product Description	The H7N7 (A/equine/Kentucky/1a/1975) HA Protein(KMP4564) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/equine/Kentucky/1a/ 1975(H7N7)) hemagglutinin (ACL12085.1) (Met1-Ile534) was expressed with a C-terminal polyhistidine tag.
Molecular Name	H7N7 HA
Species	H7N7
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA蛋白的来源和生物学意义是什么？

该蛋白源自1975年分离自美国肯塔基州马体内的H7N7禽流感病毒株，用于研究H7亚型流感病毒的宿主适应性及跨物种传播机制。
Q2-HA与HA1有何区别？

HA是完整的血凝素蛋白，包含HA1和HA2两个亚基，其中HA1负责病毒与宿主细胞受体的结合，HA2介导膜融合。本产品为全长HA蛋白。
Q3-His标签的作用是什么？如何检测？

His标签（组氨酸标签）用于简化蛋白纯化过程，可通过镍柱亲和层析纯化，并通过Western Blot或抗His抗体检测。
Q4-该蛋白适用于哪些研究？

可用于疫苗开发、中和抗体筛选、受体结合特性分析及H7N7的血清学诊断研究。
Q5-蛋白的表达系统是什么？纯度如何？

采用哺乳动物细胞表达系统，确保正确糖基化修饰，纯度≥90%（SDS-PAGE验证）。

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