H7N2 (A/ruddy turnstone/New Jersey/563/2006) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H7N2 (A/ruddy turnstone/New Jersey/563/2006) HA1 Protein, His Tag

H7N2 (A/ruddy turnstone/New Jersey/563/2006) HA1 Protein, His Tag

产品编号

KMP4563

别名

甲型流感病毒H7N2血凝素蛋白1, H7N2 HA1, Hemagglutinin Protein, H7N2 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H7N2血凝素蛋白1, H7N2 HA1, Hemagglutinin Protein, H7N2 HA1 Protein
Catalog Number	KMP4563
Product Description	The H7N2 (A/ruddy turnstone/New Jersey/563/2006) HA1 Protein(KMP4563) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/ruddy turnstone/New Jersey/563/2006(H7N2)) hemagglutinin (ACS68445.1),(Met1-Arg339), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H7N2 HA1
Species	H7N2
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 500mM NaCl, 10% Glycerol, pH7.4
Background	The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-HA1亚基在诊断试剂开发中的优势是什么？

HA1聚焦中和表位，可提高检测特异性，减少与其他H7亚型的交叉反应。
Q2-如何验证蛋白的糖基化修饰？

通过质谱分析（LC-MS/MS）鉴定N-糖基化位点，确保与天然病毒一致。
Q3-能否用于疫苗研发？

支持，作为亚单位疫苗抗原，已在小鼠模型中验证其诱导中和抗体的能力。
Q4-是否提供活性检测报告？

是的，提供血凝效价（HAU）及与特异性抗体的结合活性（EC50值）。
Q5-运输和储存条件是什么？

液体需-80℃保存。

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