H7N2 (A/ruddy turnstone/New Jersey/563/2006) HA Protein, His Tag
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产品编号
KMP4562
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别名
甲型流感病毒H7N2血凝素蛋白, H7N2 HA, Hemagglutinin Protein, H7N2 HA Protein
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规格
- 50ug
- 100ug
- 200ug
产品中心
Alias | 甲型流感病毒H7N2血凝素蛋白, H7N2 HA, Hemagglutinin Protein, H7N2 HA Protein |
Catalog Number | KMP4562 |
Product Description | The H7N2 (A/ruddy turnstone/New Jersey/563/2006) HA Protein(KMP4562) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/ruddy turnstone/New Jersey/563/2006(H7N2)) hemagglutinin (ACS68445.1) (Met1-Asp523) was expressed with a C-terminal polyhistidine tag. |
Molecular Name | H7N2 HA |
Species | H7N2 |
Host | Insect Cells |
Size | 50ug, 100ug, 200ug |
Purity | >90% as determined by SDS-PAGE |
Purification | Affinity purification |
Endotoxin | <1.0 EU/ug determined by the LAL method |
Formulation | PBS, pH7.4 |
Background | The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm. |
Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
是的,用于分析翻石鹬等迁徙鸟类中H7N2的流行情况及潜在传播风险。
采用两步纯化(His标签亲和层析+分子筛色谱),纯度≥95%,并提供质检报告。
可以,作为免疫原或检测抗原,用于小鼠或人源化抗体库的构建与筛选。
支持,可添加海藻糖或甘露醇作为冻干保护剂,延长保存期限。
液体需干冰运输,冻干粉可常温运输,附带温度记录仪。
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