H6N8 (A/mallard/Ohio/217/1998) HA Protein, His Tag_天津卡梅德科技发展有限公司

>>

>>

>>

>

H6N8 (A/mallard/Ohio/217/1998) HA Protein, His Tag

H6N8 (A/mallard/Ohio/217/1998) HA Protein, His Tag

产品编号

KMP4559

别名

甲型流感病毒H6N8血凝素蛋白, H6N8 HA, Hemagglutinin Protein, H6N8 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Alias	甲型流感病毒H6N8血凝素蛋白, H6N8 HA, Hemagglutinin Protein, H6N8 HA Protein
Catalog Number	KMP4559
Product Description	The H6N8 (A/mallard/Ohio/217/1998) HA Protein(KMP4559) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/mallard/Ohio/217/1998(H6N8)) hemagglutinin (ABO52049.1) (Met1-Gln529) was expressed with a C-terminal polyhistidine tag.
Molecular Name	H6N8 HA
Species	H6N8
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA蛋白是否适用于候鸟流感监测？

是的，作为ELISA抗原检测野鸭等候鸟血清中的H6N8特异性抗体，追踪病毒传播路径。
Q2-如何验证蛋白的天然构象？

通过ELISA检测其与广谱中和抗体（如CR8057）的结合能力，确保三聚体结构完整。
Q3-能否用于假病毒包装？

可以，与NA蛋白共表达构建假病毒，用于中和抗体或抗病毒药物筛选。
Q4-是否支持大规模生产？

支持，采用昆虫细胞悬浮培养系统，可提供克级GMP标准蛋白。
Q5-运输和储存条件是什么？

液体需-80℃保存，冻干粉可常温运输，复溶后避免反复冻融。

推荐产品

400-621-6806
614353794
周一至周五 9:00-18:00
0