H6N5 (A/shearwater/Australia/1/1973) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H6N5 (A/shearwater/Australia/1/1973) HA1 Protein, His Tag

H6N5 (A/shearwater/Australia/1/1973) HA1 Protein, His Tag

产品编号

KMP4558

别名

甲型流感病毒H6N5血凝素蛋白1, H6N5 HA1, Hemagglutinin Protein, H6N5 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H6N5血凝素蛋白1, H6N5 HA1, Hemagglutinin Protein, H6N5 HA1 Protein
Catalog Number	KMP4558
Product Description	The H6N5 (A/shearwater/Australia/1/1973) HA1 Protein(KMP4558) is produced in HEK293 Cells. A DNA sequence encoding the Influenza A virus (A/shearwater/Australia/1/1973(H6N5)) hemagglutinin (ABI84516.1) (Met1-Arg344), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H6N5 HA1
Species	H6N5
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	20mM Tris, 500mM NaCl, 10% Glycerol, pH8.0
Background	The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA1蛋白是否具有海洋鸟类流感研究的价值？

是的，用于分析剪水鹱等海鸟中H6N5的传播链及进化特征。
Q2-如何保证蛋白的高纯度？

采用两步纯化（His标签亲和层析+离子交换层析），纯度≥95%，并提供质检报告。
Q3-能否用于进化树分析？

可以，结合序列比对和抗原性分析，研究HA1的氨基酸突变对宿主适应的影响。
Q4-是否支持定制突变体（如模拟自然变异）？

支持，可引入
Q226L或G228S等突变，模拟宿主适应性进化。
Q5-运输条件是什么？

液体需干冰运输，冻干粉可常温运输，复溶后建议分装保存于-80℃。

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