H5N9 (A/chicken/Italy/22A/1998) HA Protein, His Tag_天津卡梅德科技发展有限公司

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H5N9 (A/chicken/Italy/22A/1998) HA Protein, His Tag

H5N9 (A/chicken/Italy/22A/1998) HA Protein, His Tag

产品编号

KMP4555

别名

甲型流感病毒H5N9血凝素蛋白, H5N9 HA, Hemagglutinin Protein, H5N9 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H5N9血凝素蛋白, H5N9 HA, Hemagglutinin Protein, H5N9 HA Protein
Catalog Number	KMP4555
Product Description	The H5N9 (A/chicken/Italy/22A/1998) HA Protein(KMP4555) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/chicken/Italy/22A/1998(H5N9)) hemagglutinin (ABR37720.1) (Met1-Gln527) was expressed with a C-terminal polyhistidine tag.
Molecular Name	H5N9 HA
Species	H5N9
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>95% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	The fourth gene of the EBOV genome encodes a 16-kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA蛋白是否适用于家禽疫苗研发？

是的，作为灭活疫苗或重组亚单位疫苗的候选抗原，评估其对鸡群的免疫保护效果。
Q2-如何验证蛋白的血凝活性？

通过鸡红细胞凝集试验（HAU），定量检测每μg蛋白的凝集效价。
Q3-能否用于禽流感监测？

可以，作为ELISA抗原检测禽类血清中的H5N9特异性抗体。
Q4-是否支持HA与NA蛋白的共表达？

支持，可共表达H5N9 HA与NA蛋白，用于假病毒系统构建或疫苗研究。
Q5-运输和储存条件是什么？

液体需-80℃保存，冻干粉可常温运输，复溶后分装避免反复冻融。

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