H5N8 (A/turkey/Ireland/1378/1983) HA Protein, His Tag_天津卡梅德科技发展有限公司

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H5N8 (A/turkey/Ireland/1378/1983) HA Protein, His Tag

H5N8 (A/turkey/Ireland/1378/1983) HA Protein, His Tag

产品编号

KMP4553

别名

甲型流感病毒H5N8血凝素蛋白, H5N8 HA, Hemagglutinin Protein, H5N8 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H5N8血凝素蛋白, H5N8 HA, Hemagglutinin Protein, H5N8 HA Protein
Catalog Number	KMP4553
Product Description	The H5N8 (A/turkey/Ireland/1378/1983) HA Protein(KMP4553) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/turkey/Ireland/1378/1983(H5N8)) hemagglutinin (ABI85117.1) (Met1-Gln527), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H5N8 HA
Species	H5N8
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<0.01 EU/ug determined by the LAL method
Formulation	50mM His, 0.5M NaCl, pH6.2
Background	Papillomaviruses are highly species-specific and can cause squamous epithelial and fibroepithelial tumors in their hosts. Human papillomaviruses (HPVs) are associated with benign and malignant hyperproliferation of cells, with a wide variety of clinical manifestations ranging from condyloma acuminatum to cervical carcinoma. HPV infection is the most common sexually transmitted disease. More than 4 HPV types so far identified are known to infect the genital tract. Genital HPVs are divided into `low risk' HPVs such as HPV 6 and 11 and ‘high risk’ HPV types such as 16, 18, 31, 33, 35, 39, 45 and 52, 58 which are responsible for more than 95% of HPV-induced cervical cancer. Vaccination against these high-risk types seems to be the most feasible prevention for cervical cancer. Indeed, clinical trials have shown prophylactic HPV vaccines to be effective against HPV infection, cervical intraepithelial neoplasia (CIN), and genital warts, but protection is type-specific and the currently developed vaccines target only a few types. These vaccines are based on papillomavirus-like particles (VLPs) composed of the major capsid protein, L1. The L1 protein self assembles into VLPs when expressed at high levels in eukaryotic or insect cells. VLPs are composed of 36 copies of L1 protein organized into 72 pentamers, so-called capsomeres, to form particles that are immunologically indistinguishable from native virions. Experimentally induced VLP antisera are mostly type-specific for neutralization. Minor cross-neutralization has been observed only between closely related HPV types, e.g. HPV6 and 11, HPV18 and 45, or HPV16 and 31. Structure analysis has revealed the presence of several hypervariable loops on the outer surface of the capsid. With a few exceptions, all HPV-neutralizing monoclonal antibodies analyzed so far are type-specific and recognize conformational epitopes within surface-exposed hypervariable loops of the major capsid protein L1.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA蛋白是否具有高致病性禽流感（HPAI）特征？

是的，含有多碱性切割位点，需在BSL-3实验室操作活病毒相关实验。
Q2-能否用于中和抗体检测？

可以，结合假病毒系统或微量中和实验，评估血清抗体对H5N8的中和能力。
Q3-如何控制内毒素水平？

采用无内毒素表达系统（如昆虫细胞），并通过LAL检测确保内毒素＜0.1 EU/μg。
Q4-是否支持HA三聚体的定制？

支持，通过分子伴侣共表达或结构稳定突变（如T4 fibritin标签），增强三聚体形成。
Q5-运输和储存有何特殊要求？

需全程-80℃保存，运输使用干冰，附带生物安全运输证明。

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