H3N8 (A/equine/Gansu/7/2008) HA1 Protein, His Tag_天津卡梅德科技发展有限公司

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H3N8 (A/equine/Gansu/7/2008) HA1 Protein, His Tag

H3N8 (A/equine/Gansu/7/2008) HA1 Protein, His Tag

产品编号

KMP4545

别名

甲型流感病毒H3N8血凝素蛋白1, H3N8 HA1, Hemagglutinin Protein, H3N8 HA1 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H3N8血凝素蛋白1, H3N8 HA1, Hemagglutinin Protein, H3N8 HA1 Protein
Catalog Number	KMP4545
Product Description	The H3N8 (A/equine/Gansu/7/2008) HA1 Protein(KMP4545) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/equine/Gansu/7/2008(H3N8)) hemagglutinin (ACE81938.1) (Met1-Arg344), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H3N8 HA1
Species	H3N8
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<0.01 EU/ug determined by the LAL method
Formulation	50mM His, 0.5M NaCl, pH6.2
Background	Papillomaviruses are highly species-specific and can cause squamous epithelial and fibroepithelial tumors in their hosts. Human papillomaviruses (HPVs) are associated with benign and malignant hyperproliferation of cells, with a wide variety of clinical manifestations ranging from condyloma acuminatum to cervical carcinoma. HPV infection is the most common sexually transmitted disease. More than 4 HPV types so far identified are known to infect the genital tract. Genital HPVs are divided into `low risk' HPVs such as HPV 6 and 11 and ‘high risk’ HPV types such as 16, 18, 31, 33, 35, 39, 45 and 52, 58 which are responsible for more than 95% of HPV-induced cervical cancer. Vaccination against these high-risk types seems to be the most feasible prevention for cervical cancer. Indeed, clinical trials have shown prophylactic HPV vaccines to be effective against HPV infection, cervical intraepithelial neoplasia (CIN), and genital warts, but protection is type-specific and the currently developed vaccines target only a few types. These vaccines are based on papillomavirus-like particles (VLPs) composed of the major capsid protein, L1. The L1 protein self assembles into VLPs when expressed at high levels in eukaryotic or insect cells. VLPs are composed of 36 copies of L1 protein organized into 72 pentamers, so-called capsomeres, to form particles that are immunologically indistinguishable from native virions. Experimentally induced VLP antisera are mostly type-specific for neutralization. Minor cross-neutralization has been observed only between closely related HPV types, e.g. HPV6 and 11, HPV18 and 45, or HPV16 and 31. Structure analysis has revealed the presence of several hypervariable loops on the outer surface of the capsid. With a few exceptions, all HPV-neutralizing monoclonal antibodies analyzed so far are type-specific and recognize conformational epitopes within surface-exposed hypervariable loops of the major capsid protein L1.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA1蛋白是否适用于马流感疫苗开发？

是的，作为亚单位疫苗抗原，可诱导针对H3N8亚型的中和抗体，保护马匹免受感染。
Q2-如何检测蛋白的受体结合特异性？

通过唾液酸受体结合实验（如α-2,3 vs. α-2,6连接偏好性），验证其对马呼吸道上皮的亲和力。
Q3-是否提供活性检测报告？

是的，提供血凝效价（HAU）数据及与特异性抗体的结合活性（EC50值）。
Q4-能否用于跨物种传播研究？

可以，结合犬或人类细胞模型，分析HA1的宿主适应性突变。
Q5-运输和储存条件是什么？

液体需-80℃保存，冻干粉可常温运输，复溶后分装避免反复冻融。

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