H9N2 (A/Guinea fowl/Hong Kong/WF10/99) HA Protein, His Tag_天津卡梅德科技发展有限公司

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H9N2 (A/Guinea fowl/Hong Kong/WF10/99) HA Protein, His Tag

H9N2 (A/Guinea fowl/Hong Kong/WF10/99) HA Protein, His Tag

产品编号

KMP4494

别名

甲型流感病毒H9N2血凝素蛋白, H9N2 HA, Hemagglutinin Protein, H9N2 HA Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	甲型流感病毒H9N2血凝素蛋白, H9N2 HA, Hemagglutinin Protein, H9N2 HA Protein
Catalog Number	KMP4494
Product Description	The H9N2 (A/Guinea fowl/Hong Kong/WF10/99) HA Protein(KMP4494) is produced in Insect Cells. A DNA sequence encoding the Influenza A virus (A/Guinea fowl/Hong Kong/WF10/99(H9N2)) hemagglutinin (AAO46082.1) (Met1-Lys523), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	H9N2 HA
Species	H9N2
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	PBS, pH7.4
Background	Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of the influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme that facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. The monoclonal or polyclonal antibody can be raised with protein based antigen or peptide-based antigen. Antibodies raised with protein-based antigen could have better specificity and/or binding affinity than antibodies raised with peptide based antigen, but the cost associated with the recombinant protein antigen is usually higher. Anti-influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-该HA蛋白是否适用于禽类疫苗研发？

是的，作为H9N2亚型疫苗的候选抗原，评估其对家禽的免疫保护效果。
Q2-如何控制内毒素水平？

采用无内毒素表达系统（如昆虫细胞），并通过LAL检测确保内毒素＜0.1 EU/μg。
Q3-能否用于假病毒中和实验？

可以，与NA蛋白共表达构建假病毒，评估血清抗体的中和能力。
Q4-是否提供去糖基化处理的HA蛋白？

支持，使用PNGase F去除N-糖基化，适用于结构生物学研究。
Q5-运输和储存条件是什么？

液体需-80℃保存，冻干粉可常温运输，复溶后避免反复冻融。

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