| Alias | 登革热病毒非结构蛋白5, DENV NS5, Nonstructural Protein 5, DENV NS5 Protein |
| Catalog Number | KMP4423 |
| Product Description | The DENV (strain New Guinea C) NS5 Protein(KMP4423) is produced in E. coli. A DNA sequence encoding the Dengue virus (type 2, strain New Guinea C) NS5 (AAC59275.1) (Gly2492-Gln2787) was expressed with a polyhistidine tag at the N-terminus. |
| Molecular Name | DENV NS5 |
| Species | DENV |
| Host | E. coli |
| Size | 50ug, 100ug, 200ug |
| Purity | >95% as determined by SDS-PAGE |
| Purification | Affinity purification |
| Endotoxin | <1.0 EU/ug determined by the LAL method |
| Formulation | 20mM Tris, 500mM NaCl, 10% Glycerol, pH8.0 |
| Background | Envelope of Zika virus is responsible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent with those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross-reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross-reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus. |
| Storage Condition | Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles. |
| Shipping Condition | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice. |
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