CyCMV (strain OT-1) GB Protein, Fc Tag_天津卡梅德科技发展有限公司

>>

>>

>>

>

CyCMV (strain OT-1) GB Protein, Fc Tag

CyCMV (strain OT-1) GB Protein, Fc Tag

产品编号

KMP4419

别名

巨细胞病毒巨细胞病毒包膜糖蛋白, CyCMV GB, Glycoprotein B, CyCMV GB Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Alias	巨细胞病毒巨细胞病毒包膜糖蛋白, CyCMV GB, Glycoprotein B, CyCMV GB Protein
Catalog Number	KMP4419
Product Description	The CyCMV (strain OT-1) GB Protein(KMP4419) is produced in HEK293 Cells. A DNA sequence encoding the macaca fascicularis cytomegalovirus (CyCMV)(strain OT-1) glycoprotein B(gB) (ADV35145.1) (Met1-Asp680, with cleavage site mutated ‘RHKR’ to ‘TTQT’) was expressed with the Fc region of human IgG1 at the C-terminus.
Molecular Name	CyCMV GB
Species	CyCMV
Host	HEK293 Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	40mM PB, 500mM NaCl, pH7.4
Background	Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-GB蛋白在巨细胞病毒（CMV）中的功能是什么？

GB是CMV的包膜糖蛋白复合体成员，参与病毒与宿主细胞的融合及免疫逃逸。
Q2-Fc标签是否影响蛋白的天然构象？

标签位于C端，经ELISA和SPR验证，不影响其与特异性抗体的结合能力。
Q3-能否用于移植患者CMV感染监测？

是的，作为ELISA抗原检测患者血清中的IgG/IgM抗体，评估感染风险。
Q4-如何验证蛋白的糖基化修饰？

通过PNGase F消化和质谱分析，确认N-糖基化位点与天然病毒一致。
Q5-运输和储存条件是什么？

液体需-80℃保存，冻干粉可常温运输，复溶后分装避免反复冻融。

推荐产品

400-621-6806
614353794
周一至周五 9:00-18:00
0