AcMNPV gp64 Protein, His Tag_天津卡梅德科技发展有限公司

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AcMNPV gp64 Protein, His Tag

AcMNPV gp64 Protein, His Tag

产品编号

KMP4416

别名

杆状病毒囊膜融合蛋白, AcMNPV gp64, Envelope Protein gp64, AcMNPV gp64 Protein

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Alias	杆状病毒囊膜融合蛋白, AcMNPV gp64, Envelope Protein gp64, AcMNPV gp64 Protein
Catalog Number	KMP4416
Product Description	The AcMNPV gp64 Protein(KMP4416) is produced in Insect Cells. A DNA sequence encoding the autographa californica nucleopolyhedrovirus (AcNPV)(strain E2) gp64 (NP_054158.1) (Met1-Thr481) was expressed with a polyhistidine tag at the C-terminus.
Molecular Name	AcMNPV gp64
Species	AcMNPV
Host	Insect Cells
Size	50ug, 100ug, 200ug
Purity	>90% as determined by SDS-PAGE
Purification	Affinity purification
Endotoxin	<1.0 EU/ug determined by the LAL method
Formulation	40mM PB, 500mM NaCl, pH7.4
Background	Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Shipping Condition	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. If supplied as liquid, the proteins will be shipped with dry ice.

FAQ

Q1-gp64蛋白在杆状病毒表达系统中的作用是什么？

gp64是杆状病毒的主要包膜蛋白，介导病毒与宿主细胞膜的融合，常用于增强重组蛋白的表达和分泌效率。
Q2-该蛋白能否用于基因递送载体优化？

可以，与目标基因共转染，提高外源蛋白在昆虫细胞中的表达水平。
Q3-如何验证蛋白的活性？

通过红细胞膜融合实验或假病毒系统，验证其介导膜融合的能力。
Q4-是否支持定制其他昆虫病毒包膜蛋白？

支持，需提供目标基因序列，可定制表达及纯化服务。
Q5-运输条件是什么？

液体需干冰运输，冻干粉可常温运输，复溶后建议分装保存于-80℃。

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