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Recombinant Human IL-6 Protein

Recombinant Human IL-6 Protein

产品编号

KRP674

别名

白细胞介素-6,Interleukin-6,BSF2,HSF,IFNB2

规格
  • 100ug
  • 500ug
  • 1mg
产品介绍
Catalog NumberKRP674
Alias白细胞介素-6,Interleukin-6,BSF2,HSF,IFNB2
Size100ug,500ug,1mg
Product Full Name Recombinant Human IL-6 Protein
Product Description
Molecular NameIL6
Product Introduction重组人IL-6蛋白是采用大肠杆菌表达系统生产的高纯度细胞因子,其氨基酸序列与生物活性与人体天然白介素-6高度一致。本品具备超高比活性(ED₅₀ < 1 ng/mL)与卓越纯度(>95%),内毒素含量极低,确保了实验结果的可靠性与可重复性。作为免疫与炎症反应中的核心信使分子,它是研究免疫调节、造血发育、肿瘤微环境及自身免疫性疾病的关键工具。广泛应用于体外细胞培养扩增、信号通路机制探索以及药物筛选与开发,是推动生命科学基础研究与转化医学发展的理想核心试剂。
Molecular Weight20-21 kDa
TargetIL6
Expression SystemE.coli
SpeciesHuman
Concentration联系销售经理获得最新批次的浓度
Purity >97%, determined by SDS-PAGE.
PurificationAffinity Purification
Uniprot IDP05231
Storage ConditionSamples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.
Temperature RangeStore the product under sterile conditions after opening at -80℃ for 12 months. Store the lyophilized powder at -20℃. Avoid repeated freeze-thaw cycles.
Shipping ConditionThis product is shipped on ice packs or dry ice.
Background白介素6(IL-6)是一种关键的细胞因子,在免疫防御、急性炎症和造血中发挥重要作用。针对IL-6或其受体的抗体药物已成为治疗类风湿关节炎、细胞因子风暴等疾病的重要靶向疗法。
Endotoxin<0.1 EU/μg, determined by LAL method.
Predicted Molecular Weight20.9 kDa
ApplicationWB, ELISA
ReconstitutionIt is recommended that the lyophilized human IL-6 protein be dissolved in PBS at a concentration of 100-200 μg/ml for further dilution as the working concentration.
Stability Repeated freezing and thawing is not recommended.
References1. Mansell, A. and B.J. Jenkins (2013) Cytokine Growth Factor Rev. 24:249.. Schuett, H. et al. (2009) Thromb. Haemost. 102:215.. Erta, M. et al. (2012) Int. J. Biol. Sci. 8:1254.. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.. Mihara, M. et al. (2012) Clin. Sci. (Lond.) 122:143.. Hirano, T. et al. (1986) Nature 324:73.. Kestler, D.P. et al. (1995) Blood 86:4559.. Kestler, D.P. et al. (1999) Am. J. Hematol. 61:169.. Bihl, M.P. et al. (2002) Am. J. Respir. Cell Mol. Biol. 27:48.0. Alberti, L. et al. (2005) Cancer Res. 65:2.1. Murakami, M. et al. (1993) Science 260:1808.2. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.3. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.4. Cerutti, A. et al. (1998) J. Immunol. 160:2145.
Function研究免疫调节、造血发育、肿瘤微环境及自身免疫性疾病的关键工具,广泛应用于体外细胞培养扩增、信号通路机制探索以及药物筛选与开发,是推动生命科学基础研究与转化医学发展的理想核心试剂。
Product Declaration该产品仅供科研使用,不可直接用于人体或注射。
FAQ
  • 如何储存293F细胞中表达的蛋白?

    蛋白可以在-80°C下储存以便长期保存。应分装并避免冻融循环,以保持蛋白的稳定性。

  • 使用IL-6是否需要特别注意其双面性?

    是的。IL-6具有促增殖/促分化与促炎症/促衰老的双重作用,需根据实验目的控制浓度与作用时间。长期高浓度IL-6可能引起干细胞耗竭或异常分化。

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