Human CD40L Receptor Protein, His Tag_天津卡梅德科技发展有限公司

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Human CD40L Receptor Protein, His Tag

Human CD40L Receptor Protein, His Tag

产品编号

KMP2431

别名

肿瘤坏死因子受体超家族成员 5, Tumor Necrosis Factor Receptor Superfamily member 5, CD40L

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Catalog Number	KMP2431
Alias	肿瘤坏死因子受体超家族成员 5, Tumor Necrosis Factor Receptor Superfamily member 5, CD40L
Size	50ug, 100ug, 200ug
Product Description	The Human CD40L Receptor Protein(KMP2431) is produced in HEK293 Cells and the target gene encoding Glu21-Arg193 is expressed with a 6His tag at the C-terminus.
Molecular Name	CD40
Product Introduction	CD40：TNF受体超家族跨膜蛋白，主要表达于抗原呈递细胞，通过CD40L结合介导免疫细胞间的协同作用。
Molecular Weight	19.99 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	A0A8Q3WKP3
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	CD40 is a Type I Transmembrane Glycoprotein that belongs to the TNF Receptor Superfamily. CD40 is expressed in B cells, follicular dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells, and several tumor cell lines. The extracellular domain of CD40 is characterized by Cysteine rich repeat regions. Interaction of CD40 with its ligand(CD40L) leads to aggregation of CD40 molecules, which in turn interact with cytoplasmic components to initiate signaling pathways. Several different TRAF proteins(adaptor proteins) have been identified to serves as mediators of the signal transduction.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

蛋白溶解后浓度偏低，如何调整？

低速离心：12,000 rpm, 5分钟，去除不溶物。缓冲液置换：使用超滤离心管（如10 kDa截留分子量）浓缩。
如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。

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