Mouse CD40L Protein, His Tag_天津卡梅德科技发展有限公司

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Mouse CD40L Protein, His Tag

Mouse CD40L Protein, His Tag

产品编号

KMP2429

别名

肿瘤坏死因子相关激活蛋白, Tumor Necrosis Factor Ligand Superfamily Member 5, CD154

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Catalog Number	KMP2429
Alias	肿瘤坏死因子相关激活蛋白, Tumor Necrosis Factor Ligand Superfamily Member 5, CD154
Size	50ug, 100ug, 200ug
Product Description	The Mouse CD40L Protein(KMP2429) is produced in HEK293 Cells and the target gene encoding Met112-Leu260 is expressed with a 6His tag at the N-terminus.
Molecular Name	CD40L
Product Introduction	CD40L（CD154）：一种II型跨膜蛋白，作为CD40的天然配体，在活化T细胞表面表达，参与B细胞活化和抗体产生。
Molecular Weight	17.24 kDa
Expression System	HEK293 Cells
Species	Mouse
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	P27548
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM PB, 200mM NaCl, 0.1mM EDTA, pH7.0
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	CD40 Ligand, also known as TNFSF5, CD154, is a type II transmembrane glycoprotein member of the TNF superfamily. Mature mouse CD40 Ligand consists of a 22 amino acid(aa) cytoplasmic domain, a transmembrane segment, and a 214 aa extracellular region. CD40 Ligand is expressed as a homotrimer on platelets and activated T cells and B cells. It is up-regulated following stimulation of basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds and activates CD40, which is expressed on the surface of B cells, dendritic cells,macrophages, monocytes, platelets, endothelial cells, and epithelial cells. Monomeric, dimeric, and trimeric forms of soluble CD40 Ligand bind to oligomeric CD40 on cell membranes. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何储存293F细胞中表达的蛋白？

蛋白可以在-80°C下储存以便长期保存。应分装并避免冻融循环，以保持蛋白的稳定性。
如何提高293F细胞中的蛋白表达效率？

可以通过优化质粒设计、使用高效转染试剂、优化细胞培养条件以及使用蛋白折叠伴侣或密码子优化来提高蛋白表达效率。

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