Human CD274 Protein, Fc Tag_天津卡梅德科技发展有限公司

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Human CD274 Protein, Fc Tag

Human CD274 Protein, Fc Tag

产品编号

KMP2421

别名

程序性死亡蛋白配体-1, programmed cell death 1 ligand 1, CD274

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2421
Alias	程序性死亡蛋白配体-1, programmed cell death 1 ligand 1, CD274
Size	50ug, 100ug, 200ug
Product Description	The Human CD274 Protein(KMP2421) is produced in HEK293 Cells and the target gene encoding Phe19-Thr239 is expressed with a Fc tag at the C-terminus.
Molecular Name	CD274
Product Introduction	CD274（PD-L1）：免疫球蛋白超家族（IgSF）成员，通过跨膜形式参与免疫细胞功能调控。
Molecular Weight	25.3 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	Q8IZR5
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM PB, 150mM NaCl, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	CD274, also known as B7-H1 or programmed death ligand 1(PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death-1 ligand-1(PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。
重组蛋白纯化常用的方法有哪些？

常用的纯化方法包括亲和层析（如His-Tag、GST-Tag）、离子交换层析、疏水相互作用层析（HIC）和凝胶过滤层析。具体方法取决于蛋白特性和标签类型。

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