Mouse CD244 Protein, His Tag_天津卡梅德科技发展有限公司

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Mouse CD244 Protein, His Tag

Mouse CD244 Protein, His Tag

产品编号

KMP2416

别名

自然杀伤细胞受体2B4, Natural killer cell receptor 2B4, CD244

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2416
Alias	自然杀伤细胞受体2B4, Natural killer cell receptor 2B4, CD244
Size	50ug, 100ug, 200ug
Product Description	The Mouse CD244 Protein(KMP2416) is produced in HEK293 Cells and the target gene encoding Gln20-Asn221 is expressed with a 6His tag at the C-terminus.
Molecular Name	CD244
Product Introduction	CD244（2B4）：免疫球蛋白超家族（IgSF）成员，以跨膜形式调节NK细胞和T细胞功能。
Molecular Weight	23.55 kDa
Expression System	HEK293 Cells
Species	Mouse
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	Q07763
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Natural killer cell receptor 2B4(2B4/CD244)is a 66 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 protein family. SLAM family proteins have an extracellular domain(ECD) with two or four Ig-like domains and at least two cytoplasmic immunoreceptor tyrosine-based switch motifs(ITSMs). 2B4 interacts with CD48, while other SLAM family proteins interact in a homophilic manner. The mouse 2B4 cDNA encodes a 397 amino acid(aa) precursor that includes a 19 aa signal sequence, a 207 aa ECD with one Ig-like V-type and one C2-type Ig-like domain, a 21 aa transmembrane segment, and a 150 aa cytoplasmic domain with four ITSMs. Within the ECD, mouse 2B4 shares 46% and 68% aa sequence identity with human and rat 2B4, respectively. 2B4/CD48 signaling cooperates with other receptor systems to either promote or inhibit NK and CD8+ T cell activation. The inhibitory activities are distinct from those of MHC I restricted inhibitory NK cell receptors. Ligation of 2B4 with antibodies or CD48 constructs can directly trigger inhibitory signaling or disrupt an inhibitory interaction, leading to cellular activation. 2B4 can also induce signaling through CD48.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何避免重组蛋白降解？

可在纯化过程中加入蛋白酶抑制剂、低温操作，优化储存条件（如分装冻存于-80°C），并避免反复冻融。某些情况下，可选用蛋白酶缺陷型宿主菌减少降解风险。
如何检测重组蛋白的表达和纯度？

常用SDS-PAGE、Western Blot检测表达，BCA/Bradford法测浓度，HPLC或质谱分析纯度，生物活性实验（如ELISA、酶活检测）验证功能。

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