Human CD27 Protein, Fc Tag, His Tag_天津卡梅德科技发展有限公司

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Human CD27 Protein, Fc Tag, His Tag

Human CD27 Protein, Fc Tag, His Tag

产品编号

KMP2411

别名

肿瘤坏死因子受体超家族成员 7, CD27 antigen, CD27L receptor, T-cell activation antigen CD27

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2411
Alias	肿瘤坏死因子受体超家族成员 7, CD27 antigen, CD27L receptor, T-cell activation antigen CD27
Size	50ug, 100ug, 200ug
Product Description	The Human CD27 Protein(KMP2411) is produced in HEK293 Cells and the target gene encoding Thr21-Ile192 is expressed with a Fc, 6His tag at the C-terminus.
Molecular Name	CD27
Product Introduction	CD27：TNF受体超家族成员，通过跨膜形式参与T细胞活化、增殖和记忆形成。
Molecular Weight	20.08 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
Purification	Affinity Purification
Uniprot ID	P26842
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM Tris, 150mM NaCl, pH8.0
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	CD27 antigen is also known as CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7, T14 and TNFRSF7. In humans, it is encoded by the CD27 gene. CD27 is a single-pass type I membrane protein with 3 TNFR-Cys repeats. It is a member of the TNF-receptor superfamily and is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. It plays a role in survival of activated T-cells and apoptosis through association with SIVA1.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何避免重组蛋白降解？

可在纯化过程中加入蛋白酶抑制剂、低温操作，优化储存条件（如分装冻存于-80°C），并避免反复冻融。某些情况下，可选用蛋白酶缺陷型宿主菌减少降解风险。
如何检测重组蛋白的表达和纯度？

常用SDS-PAGE、Western Blot检测表达，BCA/Bradford法测浓度，HPLC或质谱分析纯度，生物活性实验（如ELISA、酶活检测）验证功能。

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