Human CD270 Protein, His Tag_天津卡梅德科技发展有限公司

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Human CD270 Protein, His Tag

Human CD270 Protein, His Tag

产品编号

KMP2406

别名

疱疹病毒入侵介质蛋白, Tumor Necrosis Factor Receptor Superfamily Member 14, CD270

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Catalog Number	KMP2406
Alias	疱疹病毒入侵介质蛋白, Tumor Necrosis Factor Receptor Superfamily Member 14, CD270
Size	50ug, 100ug, 200ug
Product Description	The Human CD270 Protein(KMP2406) is produced in HEK293 Cells and the target gene encoding Leu39-Val202 is expressed with a 6His tag at the C-terminus.
Molecular Name	CD270
Product Introduction	CD270（HVEM）：TNF受体超家族成员，以跨膜形式参与多种免疫细胞间的信号传导。
Molecular Weight	18.17 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
Purification	Affinity Purification
Uniprot ID	F6Q0M4
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Herpesvirus entry mediator(HVEM) is a type I membrane protein in the TNF receptor superfamily, and it can both promote and inhibit T cell activity. HVEM is highly expressed on na-ve CD4+ T cells, CD8+ T memory cells, regulatory T cells, dendritic cells, monocytes, and neutrophils. It functions as a receptor for BTLA, CD160, LIGHT/TNFSF14, and Lymphotoxin-alpha. Ligation of HVEM by LIGHT triggers T cell, monocyte, and neutrophil activation and contributes to Th1 inflammation and cardiac allograft rejection. In contrast, HVEM binding to CD160 or BTLA suppresses T cell and dendritic cell activation and dampens intestinal inflammation. HVEM enhances the development of CD8+ T cell memory and Treg function. It is additionally expressed on intestinal epithelial cells, where its binding by intraepithelial lymphocyte(IEL) expressed CD160 promotes epitheilal integrity and host defense. The herpesvirus envelope glycoprotein gD, which binds HVEM to initiate membrane fusion, can antagonize both BTLA and LIGHT binding.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何避免重组蛋白降解？

可在纯化过程中加入蛋白酶抑制剂、低温操作，优化储存条件（如分装冻存于-80°C），并避免反复冻融。某些情况下，可选用蛋白酶缺陷型宿主菌减少降解风险。
如何检测重组蛋白的表达和纯度？

常用SDS-PAGE、Western Blot检测表达，BCA/Bradford法测浓度，HPLC或质谱分析纯度，生物活性实验（如ELISA、酶活检测）验证功能。

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