Human CD40L Receptor Protein, His Tag_天津卡梅德科技发展有限公司

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Human CD40L Receptor Protein, His Tag

Human CD40L Receptor Protein, His Tag

产品编号

KMP2402

别名

肿瘤坏死因子受体超家族成员 5, Tumor Necrosis Factor Receptor Superfamily member 5, CD40L

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Catalog Number	KMP2402
Alias	肿瘤坏死因子受体超家族成员 5, Tumor Necrosis Factor Receptor Superfamily member 5, CD40L
Size	50ug, 100ug, 200ug
Product Description	The Human CD40L Receptor Protein(KMP2402) is produced in HEK293 Cells and the target gene encoding Glu21-Arg193 is expressed with a 6His tag at the C-terminus.
Molecular Name	CD40
Product Introduction	CD40：TNF受体超家族跨膜蛋白，主要表达于抗原呈递细胞，通过CD40L结合介导免疫细胞间的协同作用。
Molecular Weight	19.99 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	A0A8Q3SI60
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM PB, 150mM NaCl, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	CD40 is a Type I Transmembrane Glycoprotein that belongs to the TNF Receptor Superfamily. CD40 is expressed in B cells, follicular dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells, and several tumor cell lines. The extracellular domain of CD40 is characterized by Cysteine rich repeat regions. Interaction of CD40 with its ligand(CD40L) leads to aggregation of CD40 molecules, which in turn interact with cytoplasmic components to initiate signaling pathways. Several different TRAF proteins(adaptor proteins) have been identified to serves as mediators of the signal transduction. CD40 plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何避免重组蛋白降解？

可在纯化过程中加入蛋白酶抑制剂、低温操作，优化储存条件（如分装冻存于-80°C），并避免反复冻融。某些情况下，可选用蛋白酶缺陷型宿主菌减少降解风险。
如何检测重组蛋白的表达和纯度？

常用SDS-PAGE、Western Blot检测表达，BCA/Bradford法测浓度，HPLC或质谱分析纯度，生物活性实验（如ELISA、酶活检测）验证功能。

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