Mouse CD80 Protein, Fc Tag_天津卡梅德科技发展有限公司

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Mouse CD80 Protein, Fc Tag

Mouse CD80 Protein, Fc Tag

产品编号

KMP2398

别名

T淋巴细胞活化抗原CD80, T-lymphocyte activation antigen CD80, Activation B7-1 antigen

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Catalog Number	KMP2398
Alias	T淋巴细胞活化抗原CD80, T-lymphocyte activation antigen CD80, Activation B7-1 antigen
Size	50ug, 100ug, 200ug
Product Description	The Mouse CD80 Protein(KMP2398) is produced in HEK293 Cells and the target gene encoding Val38-Lys245 is expressed with a Fc tag at the C-terminus.
Molecular Name	CD80
Product Introduction	CD80（B7-1）：免疫球蛋白超家族跨膜蛋白，作为CD28/CTLA-4的配体，在抗原呈递细胞表面表达。
Molecular Weight	23.69 kDa
Expression System	HEK293 Cells
Species	Mouse
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	Q3U4B5
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Cluster of Differentiation 80, also called B7-1, is a member of cell surface immunoglobulin superfamily which plays key, yet distinct roles in the activation of T cells. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4. Studies have shown that CTLA-4 binds mostly to CD80. The structure presents two extracellular domains: a membrane distal variable-like domain(IgV) and a membrane proximal Ig constant-like domain(IgC) along with an intracellular domain. Both IgV and IgC consist of anti-parallel beta sandwiches joined by a short linker region. CD80 is mostly expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

蛋白溶解后浓度偏低，如何调整？

低速离心：12,000 rpm, 5分钟，去除不溶物。缓冲液置换：使用超滤离心管（如10 kDa截留分子量）浓缩。
如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。

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