Human CD66a Protein, His Tag_天津卡梅德科技发展有限公司

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Human CD66a Protein, His Tag

Human CD66a Protein, His Tag

产品编号

KMP2395

别名

癌胚抗原相关细胞黏附分子1, Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1, CD66a

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2395
Alias	癌胚抗原相关细胞黏附分子1, Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1, CD66a
Size	50ug, 100ug, 200ug
Product Description	The Human CD66a Protein(KMP2395) is produced in HEK293 Cells and the target gene encoding Gln35-Gly428 is expressed with a 6His tag at the C-terminus.
Molecular Name	CD66a
Product Introduction	CD66a（CEACAM1）：癌胚抗原相关细胞黏附分子，以跨膜形式参与同源和异源细胞间相互作用。
Molecular Weight	44.13 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>90%
Purification	Affinity Purification
Uniprot ID	P11464#function
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM PB, 150mM NaCl, pH7.2
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1(CEACAM1) is a member of the Carcinoembryonic Antigen(CEA) family, which belongs to the immunoglobulin superfamily. CEACAM1 is originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it is found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. CEACAM1 mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. In addition, CEACAM1 plays a important role in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何避免重组蛋白降解？

可在纯化过程中加入蛋白酶抑制剂、低温操作，优化储存条件（如分装冻存于-80°C），并避免反复冻融。某些情况下，可选用蛋白酶缺陷型宿主菌减少降解风险。
如何检测重组蛋白的表达和纯度？

常用SDS-PAGE、Western Blot检测表达，BCA/Bradford法测浓度，HPLC或质谱分析纯度，生物活性实验（如ELISA、酶活检测）验证功能。

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