Human Gi24 Protein, Fc Tag_天津卡梅德科技发展有限公司

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Human Gi24 Protein, Fc Tag

Human Gi24 Protein, Fc Tag

产品编号

KMP2392

别名

V 集免疫调节受体, Platelet receptor Gi24, Gi24

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2392
Alias	V 集免疫调节受体, Platelet receptor Gi24, Gi24
Size	50ug, 100ug, 200ug
Product Description	The Human Gi24 Protein(KMP2392) is produced in HEK293 Cells and the target gene encoding Phe33-Ala194 is expressed with a Fc tag at the C-terminus.
Molecular Name	Gi24
Product Introduction	Gi24（VSIG4）：免疫球蛋白超家族跨膜蛋白，特异性表达于巨噬细胞，参与免疫耐受的建立。
Molecular Weight	18.14 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	Q9H7M9
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	platelet receptor Gi24 is a single-pass type I membrane protein, and located at the cell surface. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation. It is participated in the BMP signaling pathway. It also regulates the CD4-pasitive, alpha-beta T cell proliferation, and T cell cytokine production negatively. However, the protein can regulate stem cell differentiation positively.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何避免重组蛋白降解？

可在纯化过程中加入蛋白酶抑制剂、低温操作，优化储存条件（如分装冻存于-80°C），并避免反复冻融。某些情况下，可选用蛋白酶缺陷型宿主菌减少降解风险。
如何检测重组蛋白的表达和纯度？

常用SDS-PAGE、Western Blot检测表达，BCA/Bradford法测浓度，HPLC或质谱分析纯度，生物活性实验（如ELISA、酶活检测）验证功能。

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