Human CD40L Receptor Protein, Fc Tag_天津卡梅德科技发展有限公司

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Human CD40L Receptor Protein, Fc Tag

Human CD40L Receptor Protein, Fc Tag

产品编号

KMP2383

别名

肿瘤坏死因子受体超家族成员 5, Tumor Necrosis Factor Receptor Superfamily member 5, CD40L

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2383
Alias	肿瘤坏死因子受体超家族成员 5, Tumor Necrosis Factor Receptor Superfamily member 5, CD40L
Size	50ug, 100ug, 200ug
Product Description	The Human CD40L Receptor Protein(KMP2383) is produced in HEK293 Cells and the target gene encoding Glu21-Arg193 is expressed with a Fc tag at the C-terminus.
Molecular Name	CD40
Product Introduction	CD40：TNF受体超家族跨膜蛋白，主要表达于抗原呈递细胞，通过CD40L结合介导免疫细胞间的协同作用。
Molecular Weight	19.17 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	A0A0S2Z3C7
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM PB, 150mM NaCl, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	CD40 is a Type I Transmembrane Glycoprotein that belongs to the TNF Receptor Superfamily. CD40 is expressed in B cells, follicular dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells, and several tumor cell lines. The extracellular domain of CD40 is characterized by Cysteine rich repeat regions. Interaction of CD40 with its ligand(CD40L) leads to aggregation of CD40 molecules, which in turn interact with cytoplasmic components to initiate signaling pathways. Several different TRAF proteins(adaptor proteins) have been identified to serves as mediators of the signal transduction. CD40 plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。
重组蛋白纯化常用的方法有哪些？

常用的纯化方法包括亲和层析（如His-Tag、GST-Tag）、离子交换层析、疏水相互作用层析（HIC）和凝胶过滤层析。具体方法取决于蛋白特性和标签类型。

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