Human CD152 Protein, Fc Tag_天津卡梅德科技发展有限公司

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Human CD152 Protein, Fc Tag

Human CD152 Protein, Fc Tag

产品编号

KMP2376

别名

细胞毒T淋巴细胞相关抗原, Cytotoxic T-lymphocyte protein 4, CD152

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2376
Alias	细胞毒T淋巴细胞相关抗原, Cytotoxic T-lymphocyte protein 4, CD152
Size	50ug, 100ug, 200ug
Product Description	The Human CD152 Protein(KMP2376) is produced in HEK293 Cells and the target gene encoding Lys36-Asp161 is expressed with a Fc tag at the C-terminus.
Molecular Name	CD152
Product Introduction	CD152（CTLA-4）：免疫调节受体，以跨膜形式表达于T细胞，参与免疫应答的负向调控。
Molecular Weight	13.5 kDa
Expression System	HEK293 Cells
Species	Human
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	Q6GR94#function
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Cytotoxic Tlymphocyte 4(CTLA-4,CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily. Human or mouse CTLA4 cDNA encodes 223 amino acids(aa) including a 35 aa signal sequence, a 126 aa extracellular domain(ECD) with one Ig-like V-type domain, a 21 aa transmembrane(TM) sequence, and a 41 aa cytoplasmic sequence.It is widely expressed with highest levels in lymphoid tissues. CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin(Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何避免重组蛋白降解？

可在纯化过程中加入蛋白酶抑制剂、低温操作，优化储存条件（如分装冻存于-80°C），并避免反复冻融。某些情况下，可选用蛋白酶缺陷型宿主菌减少降解风险。
如何检测重组蛋白的表达和纯度？

常用SDS-PAGE、Western Blot检测表达，BCA/Bradford法测浓度，HPLC或质谱分析纯度，生物活性实验（如ELISA、酶活检测）验证功能。

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