Mouse CD28 Protein, Fc Tag, His Tag_天津卡梅德科技发展有限公司

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Mouse CD28 Protein, Fc Tag, His Tag

Mouse CD28 Protein, Fc Tag, His Tag

产品编号

KMP2374

别名

T细胞特异性表面糖蛋白CD28, CD28 molecule, Tp44

规格

50ug
100ug
200ug

DATASHEET MSDS

立即询价加入询价列表大包装定制

产品介绍

Catalog Number	KMP2374
Alias	T细胞特异性表面糖蛋白CD28, CD28 molecule, Tp44
Size	50ug, 100ug, 200ug
Product Description	The Mouse CD28 Protein(KMP2374) is produced in HEK293 Cells and the target gene encoding Asn20-Lys149 is expressed with a Fc, 6His tag at the C-terminus.
Molecular Name	CD28
Product Introduction	CD28：免疫球蛋白超家族（IgSF）成员，以跨膜形式作为重要的共刺激受体参与T细胞活化。
Molecular Weight	15.88 kDa
Expression System	HEK293 Cells
Species	Mouse
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	P31041
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM PB, 150mM NaCl, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	T-cell-specific surface glycoprotein CD28, contains an Ig-like V-type domain. CD28 is one of the proteins expressed on T cells that provide co-stimulatory signals, which are required for their activation. It is the receptor for CD80 and CD86. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells(APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. It involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

蛋白溶解后浓度偏低，如何调整？

低速离心：12,000 rpm, 5分钟，去除不溶物。缓冲液置换：使用超滤离心管（如10 kDa截留分子量）浓缩。
如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。

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