Mouse CD279 Protein, Fc Tag_天津卡梅德科技发展有限公司

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Mouse CD279 Protein, Fc Tag

Mouse CD279 Protein, Fc Tag

产品编号

KMP2368

别名

程序性死亡分子, Programmed cell death protein 1

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2368
Alias	程序性死亡分子, Programmed cell death protein 1
Size	50ug, 100ug, 200ug
Product Description	The Mouse CD279 Protein(KMP2368) is produced in HEK293 Cells and the target gene encoding Leu25-Gln167 is expressed with a Fc tag at the C-terminus.
Molecular Name	CD279
Product Introduction	CD279（PD-1）：免疫球蛋白超家族（IgSF）成员，通过跨膜形式参与T细胞功能调控。
Molecular Weight	16.97 kDa
Expression System	HEK293 Cells
Species	Mouse
Purity	>95%
Purification	Affinity Purification
Uniprot ID	Q544F3
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM Tris, 150mM NaCl, pH8.0
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Programmed Death-1(PD-1), firstly cloned from mouse T cell hybridoma 2B4.11, is one member of CD28/CTLA-4 superfamily. PD-1 belongs to type I transmembrane protein and acts as an important immunosuppressive molecule. This family also include members of CD28, CTLA-4 and ICOS.The mouse Programmed Death-1 protein, encoded by PD-1 gene, comprises four parts including a putative 20 aa signal peptide, a 149 aa extracellular region, a 21 aa transmembrane domain and a 98 aa cytoplasmic region. The cytoplamsic tail of PD-1 contains two structural motifs, an immunoreceptor tyrosine-based inhibitory motif(ITIM) and an immunoreceptor tyrosine-based switch motif(ITSM) formed by two tyrosine residues which make the difference in PD-1 signal mediating. Mouse PD-1 is expressed in thymus and shares about 69% aa sequence identity with human PD-1. Recently, programmed death-1(PD-1) with its ligands, programmed death ligand B7H1(PD-L1) and B7DC(PD-L2), was found to regulate T-cell activation and tolerance, upon ligand binding, inhibiting T-cell effector functions in an antigen-specific manner. PD-1 gene knocked out mice would induce some autoimmune diseases, which suggests that PD-1 acts as a co-inhibitory molecule actively participating in maintaining peripheral tolerance. Thus, PD-1 may be a useful target for the immunologic therapy of carcinoma,infection,autoimmune diseases as well as organ transplantation.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。
重组蛋白纯化常用的方法有哪些？

常用的纯化方法包括亲和层析（如His-Tag、GST-Tag）、离子交换层析、疏水相互作用层析（HIC）和凝胶过滤层析。具体方法取决于蛋白特性和标签类型。

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