Human CD40L Protein_天津卡梅德科技发展有限公司

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Human CD40L Protein

Human CD40L Protein

产品编号

KMP2359

别名

肿瘤坏死因子相关激活蛋白, Tumor Necrosis Factor Ligand Superfamily Member 5, CD154

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2359
Alias	肿瘤坏死因子相关激活蛋白, Tumor Necrosis Factor Ligand Superfamily Member 5, CD154
Size	50ug, 100ug, 200ug
Product Description	The Human CD40L Protein(KMP2359) is produced in E.coli and the target gene encoding Met113-Leu261 is expressed.
Molecular Name	CD40L
Product Introduction	CD40L（CD154）：一种II型跨膜蛋白，作为CD40的天然配体，在活化T细胞表面表达，参与B细胞活化和抗体产生。
Molecular Weight	16.19 kDa
Expression System	E. coli
Species	Human
Purity	>95%
SDS-PAGE
Purification	Affinity Purification
Uniprot ID	C1L3E0
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	20mM PB, 200mM NaCl, 0.1mM EDTA, pH7.0
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	CD40 Ligand(CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何避免重组蛋白降解？

可在纯化过程中加入蛋白酶抑制剂、低温操作，优化储存条件（如分装冻存于-80°C），并避免反复冻融。某些情况下，可选用蛋白酶缺陷型宿主菌减少降解风险。
如何检测重组蛋白的表达和纯度？

常用SDS-PAGE、Western Blot检测表达，BCA/Bradford法测浓度，HPLC或质谱分析纯度，生物活性实验（如ELISA、酶活检测）验证功能。

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