Mouse CD47 Protein, Fc Tag_天津卡梅德科技发展有限公司

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Mouse CD47 Protein, Fc Tag

Mouse CD47 Protein, Fc Tag

产品编号

KMP2353

别名

整联蛋白相关蛋白, Leukocyte Surface Antigen CD47, MER6

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2353
Alias	整联蛋白相关蛋白, Leukocyte Surface Antigen CD47, MER6
Size	50ug, 100ug, 200ug
Product Description	The Mouse CD47 Protein(KMP2353) is produced in HEK293 Cells and the target gene encoding Gln19-Pro158 is expressed with a Fc tag at the C-terminus.
Molecular Name	CD47
Product Introduction	CD47：免疫球蛋白超家族跨膜蛋白，广泛表达于多种细胞表面，通过结合SIRPα参与自我识别信号传递。
Molecular Weight	15.4 kDa
Expression System	HEK293 Cells
Species	Mouse
Purity	>95%
Purification	Affinity Purification
Uniprot ID	A0A2R8W6P0
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	CD47, also known as Integrin-Associated Protein(IAP) and OA3, is a glycosylated atypical member of the immunoglobulin superfamily. Mouse CD47 is an integral membrane protein that consists of a extracellular domain(ECD) with a single Ig-like domain, five membrane-spanning regions with short intervening loops, and C-terminal cytoplasmic tail. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It plays an important role in memory formation and synaptic plasticity in the hippocampus. As a receptor for SIRPA, it binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cellcell adhesion, it enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. It may play a role in membrane transport and/or integrin dependent signal transduction. It also prevents premature elimination of red blood cells.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。
重组蛋白纯化常用的方法有哪些？

常用的纯化方法包括亲和层析（如His-Tag、GST-Tag）、离子交换层析、疏水相互作用层析（HIC）和凝胶过滤层析。具体方法取决于蛋白特性和标签类型。

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