Cynomolgus CD279 Protein, His Tag_天津卡梅德科技发展有限公司

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Cynomolgus CD279 Protein, His Tag

Cynomolgus CD279 Protein, His Tag

产品编号

KMP2346

别名

程序性死亡分子, Programmed cell death protein 1

规格

50ug
100ug
200ug

DATASHEET MSDS

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产品介绍

Catalog Number	KMP2346
Alias	程序性死亡分子, Programmed cell death protein 1
Size	50ug, 100ug, 200ug
Product Description	The Cynomolgus CD279 Protein(KMP2346) is produced in HEK293 Cells and the target gene encoding Pro21-Gln167 is expressed with a 6His tag at the C-terminus.
Molecular Name	CD279
Product Introduction	CD279（PD-1）：免疫球蛋白超家族（IgSF）成员，通过跨膜形式参与T细胞功能调控。
Molecular Weight	16.83 kDa
Expression System	HEK293 Cells
Species	Cynomolgus
Purity	>95%
Purification	Affinity Purification
Uniprot ID	A4GW30
Storage Condition	Aliquot and store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.
Formulation	PBS, pH7.4
Shipping Condition	In general, the proteins are provided as lyophilized powder which are shipped at ambient temperature. They are shipped out in dry ice if supplied in liquid form.
Background	Programmed cell death protein 1(PDCD1) is a single-pass type I membrane protein and contains 1 Ig-like V-type domain. PD-1 is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PDCD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PDCD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.
Endotoxin	<1.0 EU/ug determined by the LAL method
Product Declaration	该产品仅供科研使用，不可直接用于人体或注射。

FAQ

如何提高重组蛋白的可溶性表达？

可优化表达条件（如降低诱导温度、调整IPTG浓度）、使用促溶标签（如SUMO、GST）、共表达分子伴侣，或尝试不同宿主系统（如哺乳动物细胞或昆虫细胞）以提高可溶性蛋白产量。
重组蛋白纯化常用的方法有哪些？

常用的纯化方法包括亲和层析（如His-Tag、GST-Tag）、离子交换层析、疏水相互作用层析（HIC）和凝胶过滤层析。具体方法取决于蛋白特性和标签类型。

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